Journal:

Article Title: Immune-induced epithelial to mesenchymal transition in vivo generates breast cancer stem cells

doi: 10.1158/0008-5472.CAN-08-3343

Figure Lengend Snippet: Panel A (left) shows western blot analysis of BCRP and PGP drug pumps in all cell lines. β actin was used as a loading control. Western blots are representative of three experiments for each protein. Panel A (middle) shows inhibition of growth (i.e. compared to control treated cells) of the tumor cells lines after in vitro treatment with mitoxantrone. Panel A (right) shows the apoptotic response to etoposide as assessed using flow cytometry. Each bar is expressed as the % apoptotic cells following treatment. A representative experiment is shown for MTT and apoptotic assays, both performed twice. Panel B (left) depicts the expression levels of MGMT in the tumor cell lines by real time PCR. Results are expressed as fold expression over E and represent one experiment. Panel B (right) shows the results of a clonogenic assay of the tumor cells following exposure to escalating doses of BCNU. Each data point is the mean (s.e.m.) of 3 replicates. Panel C (left) shows the results of real-time PCR analysis of the double stranded DNA repair genes. Results are expressed as fold expression over E. Panel C (right) shows the results of clonogenic assay after escalating doses of gamma irradiation. Each data point is the mean (± s.e.m.) of 3 replicate plates.

Article Snippet: DNA repair gene expression analysis was done using DNA Damage Signaling RT 2 Profiler PCR Array qPCR kit (SuperArray Bioscience, Frederick, MD) according to the manufacturer’s protocol and run on a Mx3005P thermal cycler (Stratagene, La Jolla, CA).

Techniques: Western Blot, Inhibition, In Vitro, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction, Clonogenic Assay, Irradiation